FastQCFastQC Report
Wed 11 Oct 2017
172112349_libAS2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename172112349_libAS2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21759085
Sequences flagged as poor quality0
Sequence length25-391
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTACACGACGCTCTTCCGATCTGGGGATCTGAGGGTCCAGGG864720.3974064166760689Illumina Single End PCR Primer 1 (95% over 23bp)
ACGAGTGCGTACACGACGCTCTTCCGATCTGGGATCTGAGGGTCCAGGGT527970.2426434751277455Illumina Single End PCR Primer 1 (95% over 23bp)
ATCAGACACGACACGACGCTCTTCCGATCTGGGCAGACGTGTGCTCTTCC392810.18052689256005022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGACC17200.0123081.016380
TTTTGGG4351.7501185E-521159.354290-299
CAAGCAA7402.101941E-519072.012370-379
CCAAGCA13901.9776851E-519037.71370-379
TCCTATT6503.2434713E-515509.107340-349
CAACTTC7203.6006575E-514701.344350-359
CATGGTT19103.734135E-513854.669370-379
ATTTTTC7804.6705623E-512924.258330-339
CCCAAAA4255.150285E-512452.903360-369
AACCTAC7855.203682E-512258.213370-379
TGCATAA8455.4814023E-511930.084320-329
AAGATAA7805.6268877E-511800.409290-299
GCATCAT9154.3291948E-1011568.27360-369
CGTTACG23155.4855722E-511430.851370-379
AACCTCT8906.08075E-511326.877330-339
CCAATTA3506.408137E-511201.023260-269
TAGAAAA9106.992795E-510574.394300-309
ATAATCA2407.3401316E-510500.96320-329
TAACCTA15008.636267E-59408.859370-379
TGTACAG29250.09046.98370-379
Produced by FastQC (version 0.11.2)