LOCUS       NM_002046               1421 bp    mRNA    linear   PRI 15-MAR-2015
DEFINITION  Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH),
            transcript variant 1, mRNA.
ACCESSION   NM_002046
VERSION     NM_002046.5  GI:576583510
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Bidet K, Dadlani D and Garcia-Blanco MA.
  TITLE     G3BP1, G3BP2 and CAPRIN1 are required for translation of interferon
            stimulated mRNAs and are targeted by a dengue virus non-coding RNA
  JOURNAL   PLoS Pathog. 10 (7), E1004242 (2014)
   PUBMED   24992036
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Iwai K, Shibukawa Y, Yamazaki N and Wada Y.
  TITLE     Transglutaminase 2-dependent deamidation of
            glyceraldehyde-3-phosphate dehydrogenase promotes trophoblastic
            cell fusion
  JOURNAL   J. Biol. Chem. 289 (8), 4989-4999 (2014)
   PUBMED   24375405
  REMARK    GeneRIF: TG2-dependent GAPDH deamidation was suggested to
            participate in actin cytoskeletal remodeling.
REFERENCE   3  (bases 1 to 1421)
  AUTHORS   Li T, Liu M, Feng X, Wang Z, Das I, Xu Y, Zhou X, Sun Y, Guan KL,
            Xiong Y and Lei QY.
  TITLE     Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254
            acetylation in response to glucose signal
  JOURNAL   J. Biol. Chem. 289 (6), 3775-3785 (2014)
   PUBMED   24362262
  REMARK    GeneRIF: acetylation of GAPDH (K254) is reversibly regulated by the
            acetyltransferase PCAF and the deacetylase HDAC5.
REFERENCE   4  (bases 1 to 1421)
  AUTHORS   Zhuang J, Wang PY, Huang X, Chen X, Kang JG and Hwang PM.
  TITLE     Mitochondrial disulfide relay mediates translocation of p53 and
            partitions its subcellular activity
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 110 (43), 17356-17361 (2013)
   PUBMED   24101517
REFERENCE   5  (bases 1 to 1421)
  AUTHORS   Wagener J, Schneider JJ, Baxmann S, Kalbacher H, Borelli C, Nuding
            S, Kuchler R, Wehkamp J, Kaeser MD, Mailander-Sanchez D, Braunsdorf
            C, Hube B, Schild L, Forssmann WG, Korting HC, Liepke C and
            Schaller M.
  TITLE     A peptide derived from the highly conserved protein GAPDH is
            involved in tissue protection by different antifungal strategies
            and epithelial immunomodulation
  JOURNAL   J. Invest. Dermatol. 133 (1), 144-153 (2013)
   PUBMED   22832495
  REMARK    GeneRIF: The protein encoded by this gene contains a peptide that
            displays antimicrobial activity against E. coli, P. aeruginosa, and
            C. albicans.
REFERENCE   6  (bases 1 to 1421)
  AUTHORS   Tristan C, Shahani N, Sedlak TW and Sawa A.
  TITLE     The diverse functions of GAPDH: views from different subcellular
            compartments
  JOURNAL   Cell. Signal. 23 (2), 317-323 (2011)
   PUBMED   20727968
  REMARK    GeneRIF: GAPDH is a moonlighting protein that functions as a
            glycolytic enzyme as well as a uracil DNA glycosylase.
            Review article
REFERENCE   7  (bases 1 to 1421)
  AUTHORS   Dawson SJ and White LA.
  TITLE     Treatment of Haemophilus aphrophilus endocarditis with
            ciprofloxacin
  JOURNAL   J. Infect. 24 (3), 317-320 (1992)
   PUBMED   1602151
REFERENCE   8  (bases 1 to 1421)
  AUTHORS   Meyer-Siegler K, Mauro DJ, Seal G, Wurzer J, deRiel JK and Sirover
            MA.
  TITLE     A human nuclear uracil DNA glycosylase is the 37-kDa subunit of
            glyceraldehyde-3-phosphate dehydrogenase
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88 (19), 8460-8464 (1991)
   PUBMED   1924305
REFERENCE   9  (bases 1 to 1421)
  AUTHORS   Mercer,W.D., Winn,S.I. and Watson,H.C.
  TITLE     Twinning in crystals of human skeletal muscle
            D-glyceraldehyde-3-phosphate dehydrogenase
  JOURNAL   J. Mol. Biol. 104 (1), 277-283 (1976)
   PUBMED   957435
REFERENCE   10 (bases 1 to 1421)
  AUTHORS   Nowak,K., Kuczek,M., Ostropolska,L., Malarska,A., Wolny,M. and
            Baranowski,T.
  TITLE     The covalent structure of glyceraldehyde-phosphate dehydrogenase
            from human muscles. Isolation and amino acid sequences of peptides
            from tryptic digest
  JOURNAL   Hoppe-Seyler's Z. Physiol. Chem. 356 (7), 1181-1183 (1975)
   PUBMED   1193541
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from HY046784.1, BC009081.1 and BE893087.1.
            This sequence is a reference standard in the RefSeqGene project.
            On Jan 28, 2014 this sequence version replaced gi:378404906.
            
            Summary: This gene encodes a member of the
            glyceraldehyde-3-phosphate dehydrogenase protein family. The
            encoded protein has been identified as a moonlighting protein based
            on its ability to perform mechanistically distinct functions. The
            product of this gene catalyzes an important energy-yielding step in
            carbohydrate metabolism, the reversible oxidative phosphorylation
            of glyceraldehyde-3-phosphate in the presence of inorganic
            phosphate and nicotinamide adenine dinucleotide (NAD). The encoded
            protein has additionally been identified to have uracil DNA
            glycosylase activity in the nucleus. Also, this protein contains a
            peptide that has antimicrobial activity against E. coli, P.
            aeruginosa, and C. albicans. Studies of a similar protein in mouse
            have assigned a variety of additional functions including
            nitrosylation of nuclear proteins, the regulation of mRNA
            stability, and acting as a transferrin receptor on the cell surface
            of macrophage. Many pseudogenes similar to this locus are present
            in the human genome. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s)    :: PMID: 20727968
            Protein has antimicrobial activity :: PMID: 22832495
            ##RefSeq-Attributes-END##
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF261085.1, BC013310.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               HY046784.1         10-136
            128-1397            BC009081.1         1-1270
            1398-1421           BE893087.1         178-201
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13"
     gene            1..1421
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
                     /db_xref="GeneID:2597"
                     /db_xref="HGNC:HGNC:4141"
                     /db_xref="HPRD:00713"
                     /db_xref="MIM:138400"
     exon            1..165
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             110..219
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC102046P1"
                     /db_xref="UniSTS:270066"
     STS             136..425
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC97302P2"
                     /db_xref="UniSTS:273647"
     exon            166..217
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     CDS             189..1196
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /EC_number="1.2.1.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     aging-associated gene 9 protein; peptidyl-cysteine
                     S-nitrosylase GAPDH; epididymis secretory sperm binding
                     protein Li 162eP"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase isoform
                     1"
                     /protein_id="NP_002037.2"
                     /db_xref="GI:7669492"
                     /db_xref="CCDS:CCDS8549.1"
                     /db_xref="GeneID:2597"
                     /db_xref="HGNC:HGNC:4141"
                     /db_xref="HPRD:00713"
                     /db_xref="MIM:138400"
                     /translation="MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMV
                     YMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVF
                     TTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPL
                     AKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVG
                     KVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGY
                     TEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASK
                     E"
     misc_feature    192..632
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P04406.3);
                     Region: Interaction with WARS"
     mat_peptide     192..284
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /product="hGAPDH (2-32)"
                     /experiment="DESCRIPTION:antimicrobial peptide[PMID:
                     22832495]"
     misc_feature    201..203
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    213..215
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    312..314
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphotyrosine. {ECO:0000269|PubMed:15592455};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    369..371
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000269|PubMed:19608861};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     acetylation site"
     misc_feature    378..380
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    384..386
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    396..398
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    411..413
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphothreonine. {ECO:0000269|PubMed:18183946,
                     ECO:0000269|PubMed:20068231}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); phosphorylation site"
     misc_feature    435..437
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:17081983,
                     ECO:0000269|PubMed:18669648, ECO:0000269|PubMed:20068231,
                     ECO:0000269|PubMed:21406692}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); phosphorylation site"
     misc_feature    552..554
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:18183946};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    630..632
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:18183946};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    633..635
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    639..647
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P04406.3);
                     Region: Glyceraldehyde 3-phosphate binding. {ECO:0000250}"
     misc_feature    639..641
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:18669648};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    642..644
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="ADP-ribosylcysteine, by autocatalysis, in
                     irreversibly inhibited form. {ECO:0000250}; propagated
                     from UniProtKB/Swiss-Prot (P04406.3); modified site"
     misc_feature    642..644
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="S-nitrosocysteine, in reversibly inhibited form.
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot
                     (P04406.3); nitrosylation site"
     misc_feature    642..644
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Cysteine persulfide. {ECO:0000250}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); sulfatation site"
     misc_feature    651..653
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    723..725
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Activates thiol group during catalysis; propagated
                     from UniProtKB/Swiss-Prot (P04406.3); other site"
     misc_feature    738..740
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphothreonine. {ECO:0000269|PubMed:18669648,
                     ECO:0000269|PubMed:19690332, ECO:0000269|PubMed:20068231};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    768..770
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine, alternate.
                     {ECO:0000269|PubMed:19608861}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); acetylation site"
     misc_feature    768..770
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine, alternate.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    768..770
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-malonyllysine, alternate.
                     {ECO:0000269|PubMed:21908771}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); modified site"
     misc_feature    819..824
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="propagated from UniProtKB/Swiss-Prot (P04406.3);
                     Region: Glyceraldehyde 3-phosphate binding. {ECO:0000250}"
     misc_feature    819..821
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphothreonine. {ECO:0000269|PubMed:19690332};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    831..833
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine, alternate.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    831..833
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-malonyllysine, alternate.
                     {ECO:0000269|PubMed:21908771}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); modified site"
     misc_feature    843..845
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000269|PubMed:19608861};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     acetylation site"
     misc_feature    861..863
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    867..869
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine, alternate.
                     {ECO:0000269|PubMed:19608861}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); acetylation site"
     misc_feature    867..869
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine, alternate.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    873..875
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphothreonine. {ECO:0000269|PubMed:18183946};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    897..899
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphothreonine. {ECO:0000269|PubMed:18183946};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     phosphorylation site"
     misc_feature    948..950
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6-acetyllysine. {ECO:0000269|PubMed:19608861};
                     propagated from UniProtKB/Swiss-Prot (P04406.3);
                     acetylation site"
     misc_feature    966..968
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    975..977
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     misc_feature    1122..1124
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Phosphoserine. {ECO:0000269|PubMed:18183946,
                     ECO:0000269|PubMed:19690332}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); phosphorylation site"
     misc_feature    1134..1136
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Deamidated asparagine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); amidation site"
     misc_feature    1188..1190
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="N6,N6-dimethyllysine.
                     {ECO:0000269|PubMed:18183946}; propagated from
                     UniProtKB/Swiss-Prot (P04406.3); methylation site"
     STS             194..419
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC102702P1"
                     /db_xref="UniSTS:270092"
     STS             194..419
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC351321P5"
                     /db_xref="UniSTS:273246"
     STS             195..1196
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC31284P2"
                     /db_xref="UniSTS:272922"
     STS             197..693
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC104479P2"
                     /db_xref="UniSTS:270113"
     STS             197..311
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC112827P1"
                     /db_xref="UniSTS:270244"
     STS             198..553
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC21468P1"
                     /db_xref="UniSTS:272060"
     STS             198..553
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC266773P1"
                     /db_xref="UniSTS:272348"
     STS             198..497
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC115884P2"
                     /db_xref="UniSTS:270303"
     STS             198..358
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC112330P1"
                     /db_xref="UniSTS:270229"
     STS             199..1181
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC108311P2"
                     /db_xref="UniSTS:270135"
     STS             199..556
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC114947P1"
                     /db_xref="UniSTS:270281"
     STS             201..553
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC97327P1"
                     /db_xref="UniSTS:273649"
     STS             202..567
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC108957P1"
                     /db_xref="UniSTS:270147"
     exon            218..317
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             222..593
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC164841P2"
                     /db_xref="UniSTS:271520"
     STS             227..417
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC24576P1"
                     /db_xref="UniSTS:272266"
     STS             265..1142
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="GDB:374186"
                     /db_xref="UniSTS:156947"
     STS             293..982
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC156256P2"
                     /db_xref="UniSTS:271388"
     STS             295..737
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC112647P2"
                     /db_xref="UniSTS:270238"
     STS             299..734
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC112724P1"
                     /db_xref="UniSTS:270242"
     STS             304..419
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC153210P3"
                     /db_xref="UniSTS:271253"
     exon            318..424
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             322..483
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC86881P1"
                     /db_xref="UniSTS:273552"
     STS             373..981
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC21573P1"
                     /db_xref="UniSTS:272061"
     STS             406..752
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC101742P3"
                     /db_xref="UniSTS:270065"
     STS             409..1059
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC164857P1"
                     /db_xref="UniSTS:271525"
     STS             409..980
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC141140P1"
                     /db_xref="UniSTS:270988"
     STS             409..838
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC102181P3"
                     /db_xref="UniSTS:270085"
     exon            425..515
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             426..719
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC156124P4"
                     /db_xref="UniSTS:271362"
     STS             467..1165
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC136694P1"
                     /db_xref="UniSTS:270802"
     STS             488..787
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC15575P1"
                     /db_xref="UniSTS:271350"
     STS             489..608
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC150665P1"
                     /db_xref="UniSTS:271067"
     STS             490..695
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC329392P1"
                     /db_xref="UniSTS:273149"
     STS             492..728
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC122568P2"
                     /db_xref="UniSTS:270398"
     STS             497..695
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC128235P1"
                     /db_xref="UniSTS:270574"
     STS             499..693
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="GDB:376732"
                     /db_xref="UniSTS:157065"
     exon            516..631
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             530..939
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC311067P7"
                     /db_xref="UniSTS:272878"
     STS             531..984
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC98059P1"
                     /db_xref="UniSTS:273662"
     STS             533..761
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC122612P1"
                     /db_xref="UniSTS:270402"
     STS             534..750
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC303337P3"
                     /db_xref="UniSTS:272495"
     STS             547..986
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC148872P2"
                     /db_xref="UniSTS:271025"
     STS             594..1133
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC113197P2"
                     /db_xref="UniSTS:270250"
     exon            632..713
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             640..850
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC150799P1"
                     /db_xref="UniSTS:271103"
     STS             669..982
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC135988P15"
                     /db_xref="UniSTS:270787"
     STS             670..978
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="GAPD"
                     /db_xref="UniSTS:266277"
     STS             714..1165
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="G65758"
                     /db_xref="UniSTS:225899"
     exon            714..1126
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             715..1053
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC317006P1"
                     /db_xref="UniSTS:273068"
     STS             717..1374
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC126184P3"
                     /db_xref="UniSTS:270532"
     STS             730..938
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC154635P1"
                     /db_xref="UniSTS:271315"
     STS             786..934
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC102105P1"
                     /db_xref="UniSTS:270073"
     STS             827..1175
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC109593P1"
                     /db_xref="UniSTS:270172"
     STS             857..1165
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC26839P1"
                     /db_xref="UniSTS:272355"
     STS             903..1062
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC25103P1"
                     /db_xref="UniSTS:272281"
     STS             917..1058
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC134726P2"
                     /db_xref="UniSTS:270748"
     STS             919..1165
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC133713P1"
                     /db_xref="UniSTS:270639"
     STS             1028..1154
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC119909P1"
                     /db_xref="UniSTS:270375"
     STS             1047..1304
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC123051P1"
                     /db_xref="UniSTS:270428"
     STS             1047..1304
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC97138P1"
                     /db_xref="UniSTS:273641"
     STS             1047..1127
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC156747P1"
                     /db_xref="UniSTS:271425"
     STS             1049..1172
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC309667P2"
                     /db_xref="UniSTS:272611"
     STS             1100..1327
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="D11S4602"
                     /db_xref="UniSTS:15393"
     STS             1100..1302
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC113938P1"
                     /db_xref="UniSTS:270260"
     STS             1108..1183
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC133764P1"
                     /db_xref="UniSTS:270654"
     exon            1127..1401
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /inference="alignment:Splign:1.39.8"
     STS             1239..1373
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
                     /standard_name="PMC99947P2"
                     /db_xref="UniSTS:273709"
     regulatory      1374..1379
                     /regulatory_class="polyA_signal_sequence"
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
     polyA_site      1401
                     /gene="GAPDH"
                     /gene_synonym="G3PD; GAPD; HEL-S-162eP"
ORIGIN      
        1 gcctcaagac cttgggctgg gactggctga gcctggcggg aggcggggtc cgagtcaccg
       61 cctgccgccg cgcccccggt ttctataaat tgagcccgca gcctcccgct tcgctctctg
      121 ctcctcctgt tcgacagtca gccgcatctt cttttgcgtc gccagccgag ccacatcgct
      181 cagacaccat ggggaaggtg aaggtcggag tcaacggatt tggtcgtatt gggcgcctgg
      241 tcaccagggc tgcttttaac tctggtaaag tggatattgt tgccatcaat gaccccttca
      301 ttgacctcaa ctacatggtt tacatgttcc aatatgattc cacccatggc aaattccatg
      361 gcaccgtcaa ggctgagaac gggaagcttg tcatcaatgg aaatcccatc accatcttcc
      421 aggagcgaga tccctccaaa atcaagtggg gcgatgctgg cgctgagtac gtcgtggagt
      481 ccactggcgt cttcaccacc atggagaagg ctggggctca tttgcagggg ggagccaaaa
      541 gggtcatcat ctctgccccc tctgctgatg cccccatgtt cgtcatgggt gtgaaccatg
      601 agaagtatga caacagcctc aagatcatca gcaatgcctc ctgcaccacc aactgcttag
      661 cacccctggc caaggtcatc catgacaact ttggtatcgt ggaaggactc atgaccacag
      721 tccatgccat cactgccacc cagaagactg tggatggccc ctccgggaaa ctgtggcgtg
      781 atggccgcgg ggctctccag aacatcatcc ctgcctctac tggcgctgcc aaggctgtgg
      841 gcaaggtcat ccctgagctg aacgggaagc tcactggcat ggccttccgt gtccccactg
      901 ccaacgtgtc agtggtggac ctgacctgcc gtctagaaaa acctgccaaa tatgatgaca
      961 tcaagaaggt ggtgaagcag gcgtcggagg gccccctcaa gggcatcctg ggctacactg
     1021 agcaccaggt ggtctcctct gacttcaaca gcgacaccca ctcctccacc tttgacgctg
     1081 gggctggcat tgccctcaac gaccactttg tcaagctcat ttcctggtat gacaacgaat
     1141 ttggctacag caacagggtg gtggacctca tggcccacat ggcctccaag gagtaagacc
     1201 cctggaccac cagccccagc aagagcacaa gaggaagaga gagaccctca ctgctgggga
     1261 gtccctgcca cactcagtcc cccaccacac tgaatctccc ctcctcacag ttgccatgta
     1321 gaccccttga agaggggagg ggcctaggga gccgcacctt gtcatgtacc atcaataaag
     1381 taccctgtgc tcaaccagtt aaaaaaaaaa aaaaaaaaaa a
//

